YidC-mediated membrane insertion of assembly mutants of subunit C of the F1F0 ATPase

Stefan Kol; Bryony R. Turrell; Jeanine de Keyzer; Martin van der Laan; Nico Nouwen; Arnold J. M. Driessen

doi:10.1074/jbc.M605317200

YidC-mediated membrane insertion of assembly mutants of subunit C of the F1F0 ATPase

Stefan Kol, Bryony R. Turrell, Jeanine de Keyzer, Martin van der Laan, Nico Nouwen, Arnold J. M. Driessen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that proteoliposomes containing only YidC support both the stable membrane insertion and the oligomerization of the c subunit of the F1F0 ATP synthase (F(0)c). Here we have shown that two mutants of F(0)c unable to form a functional F1F0 ATPase interact with YidC, require YidC for membrane insertion, but fail to oligomerize. These data show that oligomerization is not essential for the stable YidC- dependent membrane insertion of F(0)c consistent with a function of YidC as a membrane protein insertase.

Original language	English
Pages (from-to)	29762-29768
Number of pages	7
Journal	The Journal of Biological Chemistry
Volume	281
Issue number	40
DOIs	https://doi.org/10.1074/jbc.M605317200
Publication status	Published - 6-Oct-2006

Keywords

COLI INNER MEMBRANE
ESCHERICHIA-COLI
PROTEIN INSERTION
ADENOSINE-TRIPHOSPHATASE
SACCHAROMYCES-CEREVISIAE
F-0 COMPLEX
SYNTHASE
BIOGENESIS
SECYEG
REQUIREMENTS

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@article{92387573972a4539bbdbeb12b834e4b4,

title = "YidC-mediated membrane insertion of assembly mutants of subunit C of the F1F0 ATPase",

abstract = "YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that proteoliposomes containing only YidC support both the stable membrane insertion and the oligomerization of the c subunit of the F1F0 ATP synthase (F(0)c). Here we have shown that two mutants of F(0)c unable to form a functional F1F0 ATPase interact with YidC, require YidC for membrane insertion, but fail to oligomerize. These data show that oligomerization is not essential for the stable YidC- dependent membrane insertion of F(0)c consistent with a function of YidC as a membrane protein insertase.",

keywords = "COLI INNER MEMBRANE, ESCHERICHIA-COLI, PROTEIN INSERTION, ADENOSINE-TRIPHOSPHATASE, SACCHAROMYCES-CEREVISIAE, F-0 COMPLEX, SYNTHASE, BIOGENESIS, SECYEG, REQUIREMENTS",

author = "Stefan Kol and Turrell, {Bryony R.} and {de Keyzer}, Jeanine and {van der Laan}, Martin and Nico Nouwen and Driessen, {Arnold J. M.}",

year = "2006",

month = oct,

day = "6",

doi = "10.1074/jbc.M605317200",

language = "English",

volume = "281",

pages = "29762--29768",

journal = "The Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC",

number = "40",

}

TY - JOUR

T1 - YidC-mediated membrane insertion of assembly mutants of subunit C of the F1F0 ATPase

AU - Kol, Stefan

AU - Turrell, Bryony R.

AU - de Keyzer, Jeanine

AU - van der Laan, Martin

AU - Nouwen, Nico

AU - Driessen, Arnold J. M.

PY - 2006/10/6

Y1 - 2006/10/6

N2 - YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that proteoliposomes containing only YidC support both the stable membrane insertion and the oligomerization of the c subunit of the F1F0 ATP synthase (F(0)c). Here we have shown that two mutants of F(0)c unable to form a functional F1F0 ATPase interact with YidC, require YidC for membrane insertion, but fail to oligomerize. These data show that oligomerization is not essential for the stable YidC- dependent membrane insertion of F(0)c consistent with a function of YidC as a membrane protein insertase.

AB - YidC is a member of the OxaI family of membrane proteins that has been implicated in the membrane insertion of inner membrane proteins in Escherichia coli. We have recently demonstrated that proteoliposomes containing only YidC support both the stable membrane insertion and the oligomerization of the c subunit of the F1F0 ATP synthase (F(0)c). Here we have shown that two mutants of F(0)c unable to form a functional F1F0 ATPase interact with YidC, require YidC for membrane insertion, but fail to oligomerize. These data show that oligomerization is not essential for the stable YidC- dependent membrane insertion of F(0)c consistent with a function of YidC as a membrane protein insertase.

KW - COLI INNER MEMBRANE

KW - ESCHERICHIA-COLI

KW - PROTEIN INSERTION

KW - ADENOSINE-TRIPHOSPHATASE

KW - SACCHAROMYCES-CEREVISIAE

KW - F-0 COMPLEX

KW - SYNTHASE

KW - BIOGENESIS

KW - SECYEG

KW - REQUIREMENTS

U2 - 10.1074/jbc.M605317200

DO - 10.1074/jbc.M605317200

M3 - Article

SN - 0021-9258

VL - 281

SP - 29762

EP - 29768

JO - The Journal of Biological Chemistry

JF - The Journal of Biological Chemistry

IS - 40

ER -

YidC-mediated membrane insertion of assembly mutants of subunit C of the F1F0 ATPase

Abstract

Keywords

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