Transcriptional Regulation of Fatty Acid Biosynthesis in Lactococcus lactis

Tom H. Eckhardt; Dorota Skotnicka; Jan Kok; Oscar P. Kuipers

doi:10.1128/JB.02043-12

Transcriptional Regulation of Fatty Acid Biosynthesis in Lactococcus lactis

Tom H. Eckhardt, Dorota Skotnicka, Jan Kok, Oscar P. Kuipers^*

^*Corresponding author for this work

Molecular Genetics

Research output: Contribution to journal › Article › Academic › peer-review

29 Citations (Scopus)

Abstract

Here we study the influence of the putative fatty acid biosynthesis (FAB) regulator FabT (originally called RmaG [Llmg_1788]) on gene transcription in Lactococcus lactis MG1363. A strain with a knockout mutation of the putative regulator was constructed, and its transcriptome was compared to that of the wild-type strain. Almost all FAB genes were significantly upregulated in the knockout. Using electrophoretic mobility shift assays (EMSAs) and DNase I footprinting, the binding motif of the regulator and the binding locations in the genome were characterized. Fatty acid composition analysis revealed that a strain lacking FabT contained significantly more saturated acyl chains in its phospholipids. This observation demonstrates that the vital pathway of FAB in L. lactis is regulated by the repressor FabT.

Original language	English
Pages (from-to)	1081-1089
Number of pages	9
Journal	Journal of Bacteriology
Volume	195
Issue number	5
DOIs	https://doi.org/10.1128/JB.02043-12
Publication status	Published - Mar-2013

Keywords

MULTIPLE ANTIBIOTIC-RESISTANCE
CONTROLLED GENE-EXPRESSION
ESCHERICHIA-COLI
STREPTOCOCCUS-PNEUMONIAE
BACILLUS-SUBTILIS
OROTATE TRANSPORTER
LIPID HOMEOSTASIS
SUBSP CREMORIS
BINDING-SITES
LOW PH

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Transcriptional Regulation of Fatty Acid Biosynthesis in Lactococcus lactis
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title = "Transcriptional Regulation of Fatty Acid Biosynthesis in Lactococcus lactis",

abstract = "Here we study the influence of the putative fatty acid biosynthesis (FAB) regulator FabT (originally called RmaG [Llmg_1788]) on gene transcription in Lactococcus lactis MG1363. A strain with a knockout mutation of the putative regulator was constructed, and its transcriptome was compared to that of the wild-type strain. Almost all FAB genes were significantly upregulated in the knockout. Using electrophoretic mobility shift assays (EMSAs) and DNase I footprinting, the binding motif of the regulator and the binding locations in the genome were characterized. Fatty acid composition analysis revealed that a strain lacking FabT contained significantly more saturated acyl chains in its phospholipids. This observation demonstrates that the vital pathway of FAB in L. lactis is regulated by the repressor FabT.",

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AU - Eckhardt, Tom H.

AU - Skotnicka, Dorota

AU - Kok, Jan

AU - Kuipers, Oscar P.

PY - 2013/3

Y1 - 2013/3

N2 - Here we study the influence of the putative fatty acid biosynthesis (FAB) regulator FabT (originally called RmaG [Llmg_1788]) on gene transcription in Lactococcus lactis MG1363. A strain with a knockout mutation of the putative regulator was constructed, and its transcriptome was compared to that of the wild-type strain. Almost all FAB genes were significantly upregulated in the knockout. Using electrophoretic mobility shift assays (EMSAs) and DNase I footprinting, the binding motif of the regulator and the binding locations in the genome were characterized. Fatty acid composition analysis revealed that a strain lacking FabT contained significantly more saturated acyl chains in its phospholipids. This observation demonstrates that the vital pathway of FAB in L. lactis is regulated by the repressor FabT.

AB - Here we study the influence of the putative fatty acid biosynthesis (FAB) regulator FabT (originally called RmaG [Llmg_1788]) on gene transcription in Lactococcus lactis MG1363. A strain with a knockout mutation of the putative regulator was constructed, and its transcriptome was compared to that of the wild-type strain. Almost all FAB genes were significantly upregulated in the knockout. Using electrophoretic mobility shift assays (EMSAs) and DNase I footprinting, the binding motif of the regulator and the binding locations in the genome were characterized. Fatty acid composition analysis revealed that a strain lacking FabT contained significantly more saturated acyl chains in its phospholipids. This observation demonstrates that the vital pathway of FAB in L. lactis is regulated by the repressor FabT.

KW - MULTIPLE ANTIBIOTIC-RESISTANCE

KW - CONTROLLED GENE-EXPRESSION

KW - ESCHERICHIA-COLI

KW - STREPTOCOCCUS-PNEUMONIAE

KW - BACILLUS-SUBTILIS

KW - OROTATE TRANSPORTER

KW - LIPID HOMEOSTASIS

KW - SUBSP CREMORIS

KW - BINDING-SITES

KW - LOW PH

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DO - 10.1128/JB.02043-12

M3 - Article

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EP - 1089

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 5

ER -

Transcriptional Regulation of Fatty Acid Biosynthesis in Lactococcus lactis

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Keywords

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