The importance of immunohistochemical expression of EGFr in squamous cell carcinoma of the oral cavity treated with surgery and postoperative radiotherapy

Ernst J. Smid; T. Rianne Stoter; Elisabeth Bloemena; M. Vincent M. Lafleur; C. Rene Leemans; Isaac van der Waal; Ben J. Slotman; Johannes A. Langendijk

doi:10.1016/j.ijrobp.2006.03.011

The importance of immunohistochemical expression of EGFr in squamous cell carcinoma of the oral cavity treated with surgery and postoperative radiotherapy

Ernst J. Smid, T. Rianne Stoter, Elisabeth Bloemena, M. Vincent M. Lafleur, C. Rene Leemans, Isaac van der Waal, Ben J. Slotman, Johannes A. Langendijk^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Purpose: The aim of this study was to investigate the prognostic significance of epidermal growth factor (EGFr) expression in oral cavity squamous cell carcinoma (OCSCC) treated with curative surgery and postoperative radiotherapy.

Methods and Materials: This retrospective study included 165 OCSCC patients. The expression of EGFr was assessed on paraffin-embedded tissue of the primary tumor by immunohistochemistry using a monoclonal antibody directed against EGFr. Intensity of the EGFr expression was scored by two authors blinded for the clinical outcome.

Results: In the univariate analysis, locoregional control at 3 years (LRC) in the EGFr-negative cases was 69% compared with 77% in the EGFr-positive cases (p = 0.22). In the multivariate analysis for local control, a significant interaction was found between EGFr and overall treatment time of radiation (OTT). After stratification for EGFr expression, the OTT was of no importance in the EGFr-negative cases, whereas a significant difference in LRC was found in the EGFr-positive cases, in which the LRC after 3 years was 69% and 94% in case of an OTT of 0-42 days and > 42 days, respectively (P = 0.009; hazard ratio = 3.42; 95% confidence interval, 1.28-8.96). No significant association was found between EGFr expression and overall survival.

Conclusions: In the present study, no association was found between EGFr expression and outcome regarding locoregional control and overall survival. However, the results of the present study suggest that patients with squamous cell carcinoma of the oral cavity with high EGFr expression benefit more from a reduction of the overall treatment time of postoperative radiation than those with low EGFr expression. (c) 2006 Elsevier Inc.

Original language	English
Pages (from-to)	1323-1329
Number of pages	7
Journal	International Journal of Radiation Oncology Biology Physics
Volume	65
Issue number	5
DOIs	https://doi.org/10.1016/j.ijrobp.2006.03.011
Publication status	Published - 1-Aug-2006

Keywords

head-and-neck cancer
postoperative radiotherapy
EGFr expression
overall treatment time
GROWTH-FACTOR RECEPTOR
RANDOMIZED CONTROLLED-TRIAL
ADVANCED HEAD
RADIATION-THERAPY
NECK DISSECTION
NASOPHARYNGEAL CARCINOMA
PATHOLOGICAL FINDINGS
PROTEIN EXPRESSION
PROGNOSTIC VALUE
TGF-ALPHA

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Smid, E. J., Stoter, T. R., Bloemena, E., Lafleur, M. V. M., Leemans, C. R., van der Waal, I., Slotman, B. J., & Langendijk, J. A. (2006). The importance of immunohistochemical expression of EGFr in squamous cell carcinoma of the oral cavity treated with surgery and postoperative radiotherapy. International Journal of Radiation Oncology Biology Physics, 65(5), 1323-1329. https://doi.org/10.1016/j.ijrobp.2006.03.011

@article{b2c97f117f534ffcad5c27f5b23e6226,

title = "The importance of immunohistochemical expression of EGFr in squamous cell carcinoma of the oral cavity treated with surgery and postoperative radiotherapy",

abstract = "Purpose: The aim of this study was to investigate the prognostic significance of epidermal growth factor (EGFr) expression in oral cavity squamous cell carcinoma (OCSCC) treated with curative surgery and postoperative radiotherapy.Methods and Materials: This retrospective study included 165 OCSCC patients. The expression of EGFr was assessed on paraffin-embedded tissue of the primary tumor by immunohistochemistry using a monoclonal antibody directed against EGFr. Intensity of the EGFr expression was scored by two authors blinded for the clinical outcome.Results: In the univariate analysis, locoregional control at 3 years (LRC) in the EGFr-negative cases was 69% compared with 77% in the EGFr-positive cases (p = 0.22). In the multivariate analysis for local control, a significant interaction was found between EGFr and overall treatment time of radiation (OTT). After stratification for EGFr expression, the OTT was of no importance in the EGFr-negative cases, whereas a significant difference in LRC was found in the EGFr-positive cases, in which the LRC after 3 years was 69% and 94% in case of an OTT of 0-42 days and > 42 days, respectively (P = 0.009; hazard ratio = 3.42; 95% confidence interval, 1.28-8.96). No significant association was found between EGFr expression and overall survival.Conclusions: In the present study, no association was found between EGFr expression and outcome regarding locoregional control and overall survival. However, the results of the present study suggest that patients with squamous cell carcinoma of the oral cavity with high EGFr expression benefit more from a reduction of the overall treatment time of postoperative radiation than those with low EGFr expression. (c) 2006 Elsevier Inc.",

keywords = "head-and-neck cancer, postoperative radiotherapy, EGFr expression, overall treatment time, GROWTH-FACTOR RECEPTOR, RANDOMIZED CONTROLLED-TRIAL, ADVANCED HEAD, RADIATION-THERAPY, NECK DISSECTION, NASOPHARYNGEAL CARCINOMA, PATHOLOGICAL FINDINGS, PROTEIN EXPRESSION, PROGNOSTIC VALUE, TGF-ALPHA",

author = "Smid, {Ernst J.} and Stoter, {T. Rianne} and Elisabeth Bloemena and Lafleur, {M. Vincent M.} and Leemans, {C. Rene} and {van der Waal}, Isaac and Slotman, {Ben J.} and Langendijk, {Johannes A.}",

note = "Article J",

year = "2006",

month = aug,

day = "1",

doi = "10.1016/j.ijrobp.2006.03.011",

language = "English",

volume = "65",

pages = "1323--1329",

journal = "International Journal of Radiation Oncology Biology Physics",

issn = "0360-3016",

publisher = "ELSEVIER SCIENCE INC",

number = "5",

}

Smid, EJ, Stoter, TR, Bloemena, E, Lafleur, MVM, Leemans, CR, van der Waal, I, Slotman, BJ & Langendijk, JA 2006, 'The importance of immunohistochemical expression of EGFr in squamous cell carcinoma of the oral cavity treated with surgery and postoperative radiotherapy', International Journal of Radiation Oncology Biology Physics, vol. 65, no. 5, pp. 1323-1329. https://doi.org/10.1016/j.ijrobp.2006.03.011

The importance of immunohistochemical expression of EGFr in squamous cell carcinoma of the oral cavity treated with surgery and postoperative radiotherapy. / Smid, Ernst J.; Stoter, T. Rianne; Bloemena, Elisabeth et al.
In: International Journal of Radiation Oncology Biology Physics, Vol. 65, No. 5, 01.08.2006, p. 1323-1329.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - The importance of immunohistochemical expression of EGFr in squamous cell carcinoma of the oral cavity treated with surgery and postoperative radiotherapy

AU - Smid, Ernst J.

AU - Stoter, T. Rianne

AU - Bloemena, Elisabeth

AU - Lafleur, M. Vincent M.

AU - Leemans, C. Rene

AU - van der Waal, Isaac

AU - Slotman, Ben J.

AU - Langendijk, Johannes A.

N1 - Article J

PY - 2006/8/1

Y1 - 2006/8/1

N2 - Purpose: The aim of this study was to investigate the prognostic significance of epidermal growth factor (EGFr) expression in oral cavity squamous cell carcinoma (OCSCC) treated with curative surgery and postoperative radiotherapy.Methods and Materials: This retrospective study included 165 OCSCC patients. The expression of EGFr was assessed on paraffin-embedded tissue of the primary tumor by immunohistochemistry using a monoclonal antibody directed against EGFr. Intensity of the EGFr expression was scored by two authors blinded for the clinical outcome.Results: In the univariate analysis, locoregional control at 3 years (LRC) in the EGFr-negative cases was 69% compared with 77% in the EGFr-positive cases (p = 0.22). In the multivariate analysis for local control, a significant interaction was found between EGFr and overall treatment time of radiation (OTT). After stratification for EGFr expression, the OTT was of no importance in the EGFr-negative cases, whereas a significant difference in LRC was found in the EGFr-positive cases, in which the LRC after 3 years was 69% and 94% in case of an OTT of 0-42 days and > 42 days, respectively (P = 0.009; hazard ratio = 3.42; 95% confidence interval, 1.28-8.96). No significant association was found between EGFr expression and overall survival.Conclusions: In the present study, no association was found between EGFr expression and outcome regarding locoregional control and overall survival. However, the results of the present study suggest that patients with squamous cell carcinoma of the oral cavity with high EGFr expression benefit more from a reduction of the overall treatment time of postoperative radiation than those with low EGFr expression. (c) 2006 Elsevier Inc.

AB - Purpose: The aim of this study was to investigate the prognostic significance of epidermal growth factor (EGFr) expression in oral cavity squamous cell carcinoma (OCSCC) treated with curative surgery and postoperative radiotherapy.Methods and Materials: This retrospective study included 165 OCSCC patients. The expression of EGFr was assessed on paraffin-embedded tissue of the primary tumor by immunohistochemistry using a monoclonal antibody directed against EGFr. Intensity of the EGFr expression was scored by two authors blinded for the clinical outcome.Results: In the univariate analysis, locoregional control at 3 years (LRC) in the EGFr-negative cases was 69% compared with 77% in the EGFr-positive cases (p = 0.22). In the multivariate analysis for local control, a significant interaction was found between EGFr and overall treatment time of radiation (OTT). After stratification for EGFr expression, the OTT was of no importance in the EGFr-negative cases, whereas a significant difference in LRC was found in the EGFr-positive cases, in which the LRC after 3 years was 69% and 94% in case of an OTT of 0-42 days and > 42 days, respectively (P = 0.009; hazard ratio = 3.42; 95% confidence interval, 1.28-8.96). No significant association was found between EGFr expression and overall survival.Conclusions: In the present study, no association was found between EGFr expression and outcome regarding locoregional control and overall survival. However, the results of the present study suggest that patients with squamous cell carcinoma of the oral cavity with high EGFr expression benefit more from a reduction of the overall treatment time of postoperative radiation than those with low EGFr expression. (c) 2006 Elsevier Inc.

KW - head-and-neck cancer

KW - postoperative radiotherapy

KW - EGFr expression

KW - overall treatment time

KW - GROWTH-FACTOR RECEPTOR

KW - RANDOMIZED CONTROLLED-TRIAL

KW - ADVANCED HEAD

KW - RADIATION-THERAPY

KW - NECK DISSECTION

KW - NASOPHARYNGEAL CARCINOMA

KW - PATHOLOGICAL FINDINGS

KW - PROTEIN EXPRESSION

KW - PROGNOSTIC VALUE

KW - TGF-ALPHA

U2 - 10.1016/j.ijrobp.2006.03.011

DO - 10.1016/j.ijrobp.2006.03.011

M3 - Article

SN - 0360-3016

VL - 65

SP - 1323

EP - 1329

JO - International Journal of Radiation Oncology Biology Physics

JF - International Journal of Radiation Oncology Biology Physics

IS - 5

ER -

The importance of immunohistochemical expression of EGFr in squamous cell carcinoma of the oral cavity treated with surgery and postoperative radiotherapy

Abstract

Keywords

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