The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies

Marcella van Leeuwen; Jan Damoiseaux; Adriaan Duijvestijn; Peter Heeringa; Marion Gijbels; Menno de Winther; Jan Willem Cohen Tervaert

doi:10.1111/j.1749-6632.2009.04657.x

The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies

Marcella van Leeuwen, Jan Damoiseaux^*, Adriaan Duijvestijn, Peter Heeringa, Marion Gijbels, Menno de Winther, Jan Willem Cohen Tervaert

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

6 Citations (Scopus)

Abstract

Introduction: It is hypothesized that IgM antibodies to oxidized LDL are anti-atherogenic. Myeloperoxidase from plaque-infiltrating neutrophils catalyzes the production of hypochlorite (HOCl), which oxidizes LDL. Here we study the IgM response to HOCl-modified LDL in comparison to titers of T15 clonotypic natural antibodies. Methods: Plasma of LDLR-/- mice fed a normal chow or high-fat diet was obtained after 6 and 16 weeks. The IgM responses to HOCl-modified LDL and T15 clonotypic natural IgM antibodies were measured by ELISA. Results: The IgM levels in response to HOCl-modified LDL increased dramatically in the atherosclerotic group after introduction of the high-fat diet, but not in mice on normal chow. The natural IgM T15 clonotypic antibody titers revealed a more moderate increase during atherogenesis. Conclusion: Our results show that during atherogenesis there is a strong induction of IgM antibodies to HOCl-modified LDL particles. Whether these induced IgM antibodies are pro- or anti-atherogenic remains to be established.

Original language	English
Title of host publication	CONTEMPORARY CHALLENGES IN AUTOIMMUNITY
Editors	Y Shoenfeld, ME Gershwin
Place of Publication	MALDEN
Publisher	Blackwell Publishing
Pages	274-279
Number of pages	6
ISBN (Print)	978-1-57331-762-7
DOIs	https://doi.org/10.1111/j.1749-6632.2009.04657.x
Publication status	Published - 2009
Event	6th International Congress on Autoimmunity - , Portugal Duration: 10-Sept-2008 → 14-Sept-2008

Publication series

Name	Annals of the New York Academy of Sciences
Publisher	WILEY-BLACKWELL
Volume	1173
ISSN (Print)	0077-8923

Other

Other	6th International Congress on Autoimmunity
Country/Territory	Portugal
Period	10/09/2008 → 14/09/2008

Keywords

atherosclerosis
mouse
IgM antibodies
HOCl-modified LDL
LOW-DENSITY-LIPOPROTEIN
CORONARY-ARTERY-DISEASE
OXIDIZED LDL
IMMUNE-COMPLEXES
MYELOPEROXIDASE
EPITOPES
OXIDATION
LESIONS
MACROPHAGES
CELLS

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van Leeuwen, M., Damoiseaux, J., Duijvestijn, A., Heeringa, P., Gijbels, M., de Winther, M., & Tervaert, J. W. C. (2009). The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies. In Y. Shoenfeld, & ME. Gershwin (Eds.), CONTEMPORARY CHALLENGES IN AUTOIMMUNITY (pp. 274-279). (Annals of the New York Academy of Sciences; Vol. 1173). Blackwell Publishing. https://doi.org/10.1111/j.1749-6632.2009.04657.x

van Leeuwen, Marcella ; Damoiseaux, Jan ; Duijvestijn, Adriaan et al. / The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies. CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. editor / Y Shoenfeld ; ME Gershwin. MALDEN : Blackwell Publishing, 2009. pp. 274-279 (Annals of the New York Academy of Sciences).

@inproceedings{265d1fce3da74a61b68a3c27b64fd804,

title = "The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies",

abstract = "Introduction: It is hypothesized that IgM antibodies to oxidized LDL are anti-atherogenic. Myeloperoxidase from plaque-infiltrating neutrophils catalyzes the production of hypochlorite (HOCl), which oxidizes LDL. Here we study the IgM response to HOCl-modified LDL in comparison to titers of T15 clonotypic natural antibodies. Methods: Plasma of LDLR-/- mice fed a normal chow or high-fat diet was obtained after 6 and 16 weeks. The IgM responses to HOCl-modified LDL and T15 clonotypic natural IgM antibodies were measured by ELISA. Results: The IgM levels in response to HOCl-modified LDL increased dramatically in the atherosclerotic group after introduction of the high-fat diet, but not in mice on normal chow. The natural IgM T15 clonotypic antibody titers revealed a more moderate increase during atherogenesis. Conclusion: Our results show that during atherogenesis there is a strong induction of IgM antibodies to HOCl-modified LDL particles. Whether these induced IgM antibodies are pro- or anti-atherogenic remains to be established.",

keywords = "atherosclerosis, mouse, IgM antibodies, HOCl-modified LDL, LOW-DENSITY-LIPOPROTEIN, CORONARY-ARTERY-DISEASE, OXIDIZED LDL, IMMUNE-COMPLEXES, MYELOPEROXIDASE, EPITOPES, OXIDATION, LESIONS, MACROPHAGES, CELLS",

author = "{van Leeuwen}, Marcella and Jan Damoiseaux and Adriaan Duijvestijn and Peter Heeringa and Marion Gijbels and {de Winther}, Menno and Tervaert, {Jan Willem Cohen}",

year = "2009",

doi = "10.1111/j.1749-6632.2009.04657.x",

language = "English",

isbn = "978-1-57331-762-7",

series = "Annals of the New York Academy of Sciences",

publisher = "Blackwell Publishing",

pages = "274--279",

editor = "Y Shoenfeld and ME Gershwin",

booktitle = "CONTEMPORARY CHALLENGES IN AUTOIMMUNITY",

note = "6th International Congress on Autoimmunity ; Conference date: 10-09-2008 Through 14-09-2008",

}

van Leeuwen, M, Damoiseaux, J, Duijvestijn, A, Heeringa, P, Gijbels, M, de Winther, M & Tervaert, JWC 2009, The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies. in Y Shoenfeld & ME Gershwin (eds), CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. Annals of the New York Academy of Sciences, vol. 1173, Blackwell Publishing, MALDEN, pp. 274-279, 6th International Congress on Autoimmunity, Portugal, 10/09/2008. https://doi.org/10.1111/j.1749-6632.2009.04657.x

The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies. / van Leeuwen, Marcella; Damoiseaux, Jan; Duijvestijn, Adriaan et al.
CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. ed. / Y Shoenfeld; ME Gershwin. MALDEN: Blackwell Publishing, 2009. p. 274-279 (Annals of the New York Academy of Sciences; Vol. 1173).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies

AU - van Leeuwen, Marcella

AU - Damoiseaux, Jan

AU - Duijvestijn, Adriaan

AU - Heeringa, Peter

AU - Gijbels, Marion

AU - de Winther, Menno

AU - Tervaert, Jan Willem Cohen

PY - 2009

Y1 - 2009

N2 - Introduction: It is hypothesized that IgM antibodies to oxidized LDL are anti-atherogenic. Myeloperoxidase from plaque-infiltrating neutrophils catalyzes the production of hypochlorite (HOCl), which oxidizes LDL. Here we study the IgM response to HOCl-modified LDL in comparison to titers of T15 clonotypic natural antibodies. Methods: Plasma of LDLR-/- mice fed a normal chow or high-fat diet was obtained after 6 and 16 weeks. The IgM responses to HOCl-modified LDL and T15 clonotypic natural IgM antibodies were measured by ELISA. Results: The IgM levels in response to HOCl-modified LDL increased dramatically in the atherosclerotic group after introduction of the high-fat diet, but not in mice on normal chow. The natural IgM T15 clonotypic antibody titers revealed a more moderate increase during atherogenesis. Conclusion: Our results show that during atherogenesis there is a strong induction of IgM antibodies to HOCl-modified LDL particles. Whether these induced IgM antibodies are pro- or anti-atherogenic remains to be established.

AB - Introduction: It is hypothesized that IgM antibodies to oxidized LDL are anti-atherogenic. Myeloperoxidase from plaque-infiltrating neutrophils catalyzes the production of hypochlorite (HOCl), which oxidizes LDL. Here we study the IgM response to HOCl-modified LDL in comparison to titers of T15 clonotypic natural antibodies. Methods: Plasma of LDLR-/- mice fed a normal chow or high-fat diet was obtained after 6 and 16 weeks. The IgM responses to HOCl-modified LDL and T15 clonotypic natural IgM antibodies were measured by ELISA. Results: The IgM levels in response to HOCl-modified LDL increased dramatically in the atherosclerotic group after introduction of the high-fat diet, but not in mice on normal chow. The natural IgM T15 clonotypic antibody titers revealed a more moderate increase during atherogenesis. Conclusion: Our results show that during atherogenesis there is a strong induction of IgM antibodies to HOCl-modified LDL particles. Whether these induced IgM antibodies are pro- or anti-atherogenic remains to be established.

KW - atherosclerosis

KW - mouse

KW - IgM antibodies

KW - HOCl-modified LDL

KW - LOW-DENSITY-LIPOPROTEIN

KW - CORONARY-ARTERY-DISEASE

KW - OXIDIZED LDL

KW - IMMUNE-COMPLEXES

KW - MYELOPEROXIDASE

KW - EPITOPES

KW - OXIDATION

KW - LESIONS

KW - MACROPHAGES

KW - CELLS

U2 - 10.1111/j.1749-6632.2009.04657.x

DO - 10.1111/j.1749-6632.2009.04657.x

M3 - Conference contribution

SN - 978-1-57331-762-7

T3 - Annals of the New York Academy of Sciences

SP - 274

EP - 279

BT - CONTEMPORARY CHALLENGES IN AUTOIMMUNITY

A2 - Shoenfeld, Y

A2 - Gershwin, ME

PB - Blackwell Publishing

CY - MALDEN

T2 - 6th International Congress on Autoimmunity

Y2 - 10 September 2008 through 14 September 2008

ER -

van Leeuwen M, Damoiseaux J, Duijvestijn A, Heeringa P, Gijbels M, de Winther M et al. The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies. In Shoenfeld Y, Gershwin ME, editors, CONTEMPORARY CHALLENGES IN AUTOIMMUNITY. MALDEN: Blackwell Publishing. 2009. p. 274-279. (Annals of the New York Academy of Sciences). doi: 10.1111/j.1749-6632.2009.04657.x

The IgM Response to Modified LDL in Experimental Atherosclerosis Hypochlorite-modified LDL IgM Antibodies versus Classical Natural T15 IgM Antibodies

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