Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies

Giulio Abagnale; Antonio Sechi; Michael Steger; Qihui Zhou; Chao-Chung Kuo; Gülcan Aydin; Carmen Schalla; Gerhard Müller-Newen; Martin Zenke; Ivan G Costa; Patrick van Rijn; Arnold Gillner; Wolfgang Wagner

doi:10.1016/j.stemcr.2017.06.016

Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies

Giulio Abagnale, Antonio Sechi, Michael Steger, Qihui Zhou, Chao-Chung Kuo, Gülcan Aydin, Carmen Schalla, Gerhard Müller-Newen, Martin Zenke, Ivan G Costa, Patrick van Rijn, Arnold Gillner, Wolfgang Wagner^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The relevance of topographic cues for commitment of induced pluripotent stem cells (iPSCs) is largely unknown. In this study, we demonstrate that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, and direct the polarity of cell division. Elongation of iPSC colonies impacts also on their intrinsic molecular patterning, which seems to be orchestrated from the rim of the colonies. BMP4-induced differentiation is enhanced in elongated colonies, and the submicron grooves impact on the spatial modulation of YAP activity upon induction with this morphogen. Interestingly, TAZ, a YAP paralog, shows distinct cytoskeletal localization in iPSCs. These findings demonstrate that topography can guide orientation and organization of iPSC colonies, which may affect the interaction between mechanosensors and mechanotransducers in iPSCs.

Original language	English
Pages (from-to)	654-666
Number of pages	13
Journal	Stem Cell Reports
Volume	9
Issue number	2
DOIs	https://doi.org/10.1016/j.stemcr.2017.06.016
Publication status	Published - 8-Aug-2017

Keywords

HIPPO SIGNALING PATHWAY
MULTI-BEAM-INTERFERENCE
ORGAN SIZE CONTROL
SELF-RENEWAL
CONTACT GUIDANCE
TISSUE-CELLS
DIFFERENTIATION
YAP
ADHESION
LINEAGE

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title = "Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies",

abstract = "The relevance of topographic cues for commitment of induced pluripotent stem cells (iPSCs) is largely unknown. In this study, we demonstrate that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, and direct the polarity of cell division. Elongation of iPSC colonies impacts also on their intrinsic molecular patterning, which seems to be orchestrated from the rim of the colonies. BMP4-induced differentiation is enhanced in elongated colonies, and the submicron grooves impact on the spatial modulation of YAP activity upon induction with this morphogen. Interestingly, TAZ, a YAP paralog, shows distinct cytoskeletal localization in iPSCs. These findings demonstrate that topography can guide orientation and organization of iPSC colonies, which may affect the interaction between mechanosensors and mechanotransducers in iPSCs.",

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author = "Giulio Abagnale and Antonio Sechi and Michael Steger and Qihui Zhou and Chao-Chung Kuo and G{\"u}lcan Aydin and Carmen Schalla and Gerhard M{\"u}ller-Newen and Martin Zenke and Costa, {Ivan G} and {van Rijn}, Patrick and Arnold Gillner and Wolfgang Wagner",

year = "2017",

month = aug,

day = "8",

doi = "10.1016/j.stemcr.2017.06.016",

language = "English",

volume = "9",

pages = "654--666",

journal = "Stem Cell Reports",

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publisher = "Cell Press",

number = "2",

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T1 - Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies

AU - Abagnale, Giulio

AU - Sechi, Antonio

AU - Steger, Michael

AU - Zhou, Qihui

AU - Kuo, Chao-Chung

AU - Aydin, Gülcan

AU - Schalla, Carmen

AU - Müller-Newen, Gerhard

AU - Zenke, Martin

AU - Costa, Ivan G

AU - van Rijn, Patrick

AU - Gillner, Arnold

AU - Wagner, Wolfgang

PY - 2017/8/8

Y1 - 2017/8/8

N2 - The relevance of topographic cues for commitment of induced pluripotent stem cells (iPSCs) is largely unknown. In this study, we demonstrate that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, and direct the polarity of cell division. Elongation of iPSC colonies impacts also on their intrinsic molecular patterning, which seems to be orchestrated from the rim of the colonies. BMP4-induced differentiation is enhanced in elongated colonies, and the submicron grooves impact on the spatial modulation of YAP activity upon induction with this morphogen. Interestingly, TAZ, a YAP paralog, shows distinct cytoskeletal localization in iPSCs. These findings demonstrate that topography can guide orientation and organization of iPSC colonies, which may affect the interaction between mechanosensors and mechanotransducers in iPSCs.

AB - The relevance of topographic cues for commitment of induced pluripotent stem cells (iPSCs) is largely unknown. In this study, we demonstrate that groove-ridge structures with a periodicity in the submicrometer range induce elongation of iPSC colonies, guide the orientation of apical actin fibers, and direct the polarity of cell division. Elongation of iPSC colonies impacts also on their intrinsic molecular patterning, which seems to be orchestrated from the rim of the colonies. BMP4-induced differentiation is enhanced in elongated colonies, and the submicron grooves impact on the spatial modulation of YAP activity upon induction with this morphogen. Interestingly, TAZ, a YAP paralog, shows distinct cytoskeletal localization in iPSCs. These findings demonstrate that topography can guide orientation and organization of iPSC colonies, which may affect the interaction between mechanosensors and mechanotransducers in iPSCs.

KW - HIPPO SIGNALING PATHWAY

KW - MULTI-BEAM-INTERFERENCE

KW - ORGAN SIZE CONTROL

KW - SELF-RENEWAL

KW - CONTACT GUIDANCE

KW - TISSUE-CELLS

KW - DIFFERENTIATION

KW - YAP

KW - ADHESION

KW - LINEAGE

U2 - 10.1016/j.stemcr.2017.06.016

DO - 10.1016/j.stemcr.2017.06.016

M3 - Article

C2 - 28757164

SN - 2213-6711

VL - 9

SP - 654

EP - 666

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 2

ER -

Surface Topography Guides Morphology and Spatial Patterning of Induced Pluripotent Stem Cell Colonies

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Keywords

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