Sulphoxidation reactions catalysed by the Baeyer-Villiger monooxygenase OTEMO from Pseudomonas putida ATCC 17453

Gonzalo de Gonzalo*, Nikola Loncar, Marco Fraaije

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)
88 Downloads (Pure)

Abstract

The Baeyer-Villiger monooxygenase OTEMO from Pseudomonas putida ATCC 17453 has been employed as biocatalyst in the asymmetric synthesis of a set of optically active sulphoxides. Several alkyl aryl sulphides are oxidized by this biocatalyst leading to the (S)-sulphoxides. Especially for those substrates containing electron-donating groups in the aromatic ring or in the alkyl moiety, good to high enantiopurities can be obtained. OTEMO is also able to perform the kinetic resolution of racemic sulphoxides, but with low enantioselectivities. Finally, parameters that can affect its biocatalytic properties, such as pH, temperature, organic cosolvents and substrate concentration, have been tested to get a better insight into the biocatalytic potential of this hitherto poorly explored oxidative biocatalyst.

Original languageEnglish
Article number2113519
Pages (from-to)77-84
Number of pages8
JournalBiocatalysis and Biotransformation
Volume42
Issue number1
Early online date29-Aug-2022
DOIs
Publication statusPublished - 2024

Keywords

  • Baeyer-Villiger monooxygenases
  • Biocatalysis
  • kinetic resolution
  • sulphoxidations

Fingerprint

Dive into the research topics of 'Sulphoxidation reactions catalysed by the Baeyer-Villiger monooxygenase OTEMO from Pseudomonas putida ATCC 17453'. Together they form a unique fingerprint.

Cite this