TY - GEN
T1 - Real-time monitoring of biliary transport in precision-cut liver slices
AU - Grajewski, Maciej
AU - Salentijn, Gert I.J.
AU - Karsten, Ruby E.H.
AU - Olinga, Peter
AU - Verpoorte, Elisabeth
N1 - Funding Information:
This work was carried out within TisuMR project (Contract number 634130) funded by European Commission.
Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2018
Y1 - 2018
N2 - We present a polycarbonate (PC) microfluidic perfusion device for the cultivation of murine, precision-cut liver slices (PCLS). The design dimensions of this system are such that it is compatible with fluorescence microscopy for the observation of tissue and detection of fluorescently labeled markers. This system can thus be used for real-time monitoring of the uptake, metabolism and secretion of molecules by liver tissue from continuously flowing medium.
AB - We present a polycarbonate (PC) microfluidic perfusion device for the cultivation of murine, precision-cut liver slices (PCLS). The design dimensions of this system are such that it is compatible with fluorescence microscopy for the observation of tissue and detection of fluorescently labeled markers. This system can thus be used for real-time monitoring of the uptake, metabolism and secretion of molecules by liver tissue from continuously flowing medium.
KW - Fluorescence microscopy
KW - Organ-on-a-chip
KW - Real-time monitoring
UR - http://www.scopus.com/inward/record.url?scp=85079807346&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079807346
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 1644
EP - 1646
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -