TY - JOUR
T1 - Postaggregative Differentiation Induction by Cyclic AMP in Dictyostelium
T2 - Intracellular Transduction Pathway and Requirement for Additional Stimuli
AU - Schaap, Pauline
AU - Lookeren Campagne, Michiel M. van
AU - Driel, Roel van
AU - Spek, Wouter
AU - Haastert, Peter J.M. van
AU - Pinas, Johan
N1 - Relation: http://www.rug.nl/gbb/
date_submitted:2007
Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute
PY - 1986
Y1 - 1986
N2 - Cyclic AMP induces postaggregative differentiation in aggregation competent cells of Dictyostelium by interacting with cell surface cAMP receptors. We investigated the transduction pathway of this response and additional requirements for the induction of postaggregative differentiation. Optimal induction of postaggregative gene expression requires that vegetative cells are first exposed to 2-4 hr of nanomolar cAMP pulses, and subsequently for 4-6 hr to steady-state cAMP concentrations in the micromolar range. Cyclic AMP pulses, which are endogenously produced before and during aggregation, induce full reponsiveness to cAMP as a morphogen. The transduction pathway from the cell surface cAMP receptor to postaggregative gene expression may involve Ca2+ ions as intracellular messengers. A cAMP-induced increase in intracellular cAMP or cGMP levels is not involved in the transduction pathway.
AB - Cyclic AMP induces postaggregative differentiation in aggregation competent cells of Dictyostelium by interacting with cell surface cAMP receptors. We investigated the transduction pathway of this response and additional requirements for the induction of postaggregative differentiation. Optimal induction of postaggregative gene expression requires that vegetative cells are first exposed to 2-4 hr of nanomolar cAMP pulses, and subsequently for 4-6 hr to steady-state cAMP concentrations in the micromolar range. Cyclic AMP pulses, which are endogenously produced before and during aggregation, induce full reponsiveness to cAMP as a morphogen. The transduction pathway from the cell surface cAMP receptor to postaggregative gene expression may involve Ca2+ ions as intracellular messengers. A cAMP-induced increase in intracellular cAMP or cGMP levels is not involved in the transduction pathway.
U2 - 10.1016/0012-1606(86)90072-2
DO - 10.1016/0012-1606(86)90072-2
M3 - Article
VL - 118
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -