Overexpression of alcohol oxidase in Pichia pastoris

M.J. de Hoop; J. Cregg; I. Keizer-Gunnink; Klaas Sjollema; M. Veenhuis; G. Ab

doi:10.1016/0014-5793(91)81306-S

Overexpression of alcohol oxidase in Pichia pastoris

M.J. de Hoop, J. Cregg, I. Keizer-Gunnink, Klaas Sjollema, M. Veenhuis, G. Ab

Groningen Biomolecular Sciences and Biotechnology Institute

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Abstract

The protein import capacity of peroxisomes in methylotrophic yeasts was studied using Pichia pastoris containing one or two extra copies of the gene encoding the peroxisomal protein alcohol oxidase. The alcohol oxidase overproduced in this strain was only partially imported and assembled into the active, octameric form of the protein. The excess remained in the cytosol as protein aggregates composed of monomers. These results are discussed in view of the possible application of peroxisomes as storage compartments for heterologous proteins.

Original language	English
Pages (from-to)	299-302
Number of pages	4
Journal	FEBS Letters
Volume	291
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)81306-S
Publication status	Published - 21-Oct-1991

Keywords

ALCOHOL OXIDASE
METHYLOTROPHIC YEAST
PEROXISOME
GROWN HANSENULA-POLYMORPHA
METHANOL OXIDASE
PEROXISOMES
PROTEIN
MICROBODIES
YEASTS

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title = "Overexpression of alcohol oxidase in Pichia pastoris",

abstract = "The protein import capacity of peroxisomes in methylotrophic yeasts was studied using Pichia pastoris containing one or two extra copies of the gene encoding the peroxisomal protein alcohol oxidase. The alcohol oxidase overproduced in this strain was only partially imported and assembled into the active, octameric form of the protein. The excess remained in the cytosol as protein aggregates composed of monomers. These results are discussed in view of the possible application of peroxisomes as storage compartments for heterologous proteins.",

keywords = "ALCOHOL OXIDASE, METHYLOTROPHIC YEAST, PEROXISOME, GROWN HANSENULA-POLYMORPHA, METHANOL OXIDASE, PEROXISOMES, PROTEIN, MICROBODIES, YEASTS",

author = "Hoop, {M.J. de} and J. Cregg and I. Keizer-Gunnink and Klaas Sjollema and M. Veenhuis and G. Ab",

note = "Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute",

year = "1991",

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doi = "10.1016/0014-5793(91)81306-S",

language = "English",

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T1 - Overexpression of alcohol oxidase in Pichia pastoris

AU - Hoop, M.J. de

AU - Cregg, J.

AU - Keizer-Gunnink, I.

AU - Sjollema, Klaas

AU - Veenhuis, M.

AU - Ab, G.

N1 - Relation: http://www.rug.nl/gbb/ date_submitted:2007 Rights: University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute

PY - 1991/10/21

Y1 - 1991/10/21

N2 - The protein import capacity of peroxisomes in methylotrophic yeasts was studied using Pichia pastoris containing one or two extra copies of the gene encoding the peroxisomal protein alcohol oxidase. The alcohol oxidase overproduced in this strain was only partially imported and assembled into the active, octameric form of the protein. The excess remained in the cytosol as protein aggregates composed of monomers. These results are discussed in view of the possible application of peroxisomes as storage compartments for heterologous proteins.

AB - The protein import capacity of peroxisomes in methylotrophic yeasts was studied using Pichia pastoris containing one or two extra copies of the gene encoding the peroxisomal protein alcohol oxidase. The alcohol oxidase overproduced in this strain was only partially imported and assembled into the active, octameric form of the protein. The excess remained in the cytosol as protein aggregates composed of monomers. These results are discussed in view of the possible application of peroxisomes as storage compartments for heterologous proteins.

KW - ALCOHOL OXIDASE

KW - METHYLOTROPHIC YEAST

KW - PEROXISOME

KW - GROWN HANSENULA-POLYMORPHA

KW - METHANOL OXIDASE

KW - PEROXISOMES

KW - PROTEIN

KW - MICROBODIES

KW - YEASTS

U2 - 10.1016/0014-5793(91)81306-S

DO - 10.1016/0014-5793(91)81306-S

M3 - Article

SN - 0014-5793

VL - 291

SP - 299

EP - 302

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Overexpression of alcohol oxidase in Pichia pastoris

Abstract

Keywords

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