MICROWAVE STABILIZATION AND MICROWAVE-STIMULATED FIXATION

ME BOON*, LP KOK

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In microwave stabilization the tissue is prepared for histoprocessing through the uniformly distributed increased temperature. This procedure can be called unique: Exclusively by the uniform well-controlled temperature rise to 55-degrees-C it is possible to stabilize the tissue to the needed perfection, leaving the majority of antigens intact.

In microwave-stimulated fixation the chemical processes involved in the fixation procedure can be accelerated in a controlled fashion (55-degrees-C). The results depend on the fixation agent: Formaldehyde gives an optimized formaldehyde pattern, and Kryofix(R) gives a Kryofix(R) pattern with many antigens intact. Standardization of microscopic results can be achieved, and the tissue can be histoprocessed one hour after operation.

Original languageEnglish
Pages (from-to)851-854
Number of pages4
JournalInstitute of physics conference series
Issue number98
Publication statusPublished - 1990

Keywords

  • ELECTRON-MICROSCOPY
  • RAT-BRAIN
  • IRRADIATION
  • TISSUE
  • PRESERVATION
  • ANTIGENS
  • LIGHT
  • CELLS
  • HISTOCHEMISTRY
  • FORMALDEHYDE

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