Methods and means for regulating gene expression

Christiaan Daniel den Hengst; Olivera Gajic; Oscar Paul Kuipers; Jan Kok; Jan Sikkema; Johannes Marie Wilhelmus Geurts; Arjen Nauta

Methods and means for regulating gene expression

Christiaan Daniel den Hengst (Inventor), Olivera Gajic (Inventor), Oscar Paul Kuipers (Inventor), Jan Kok (Inventor), Jan Sikkema (Inventor), Johannes Marie Wilhelmus Geurts (Inventor), Arjen Nauta (Inventor)

Molecular Genetics

Research output: Patent

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Abstract

The invention relates to the field of biochemistry, molecular biology and food production. More in particular, the invention relates to methods and means for regulating gene expression. Even more in particular, the invention relates to CodY target sequences. Herein we show that CodY represses its target genes by binding to specific DNA sequences upstream of the respective genes. A conserved target site was identified by analyzing upstream sequences of derepressed genes in a delta codY L. lactis MG1363 derivative, as identified in a DNA micro-array study. The present application furthermore discloses CodY target sequences from other gram-positive bacteria, like B. subtilis and Streptococcus. Hence, the invention provides CodY target sequences that may be used in different applications to repress or derepress gene expression.

Original language	English
Patent number	WO2005003354
IPC	C12N 15/ 74 A I
Priority date	02/07/2003
Filing date	02/07/2004
Publication status	Published - 13-Jan-2005

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title = "Methods and means for regulating gene expression",

abstract = "The invention relates to the field of biochemistry, molecular biology and food production. More in particular, the invention relates to methods and means for regulating gene expression. Even more in particular, the invention relates to CodY target sequences. Herein we show that CodY represses its target genes by binding to specific DNA sequences upstream of the respective genes. A conserved target site was identified by analyzing upstream sequences of derepressed genes in a delta codY L. lactis MG1363 derivative, as identified in a DNA micro-array study. The present application furthermore discloses CodY target sequences from other gram-positive bacteria, like B. subtilis and Streptococcus. Hence, the invention provides CodY target sequences that may be used in different applications to repress or derepress gene expression.",

author = "{den Hengst}, {Christiaan Daniel} and Olivera Gajic and Kuipers, {Oscar Paul} and Jan Kok and Jan Sikkema and Geurts, {Johannes Marie Wilhelmus} and Arjen Nauta",

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T1 - Methods and means for regulating gene expression

AU - den Hengst, Christiaan Daniel

AU - Gajic, Olivera

AU - Kuipers, Oscar Paul

AU - Kok, Jan

AU - Sikkema, Jan

AU - Geurts, Johannes Marie Wilhelmus

AU - Nauta, Arjen

PY - 2005/1/13

Y1 - 2005/1/13

N2 - The invention relates to the field of biochemistry, molecular biology and food production. More in particular, the invention relates to methods and means for regulating gene expression. Even more in particular, the invention relates to CodY target sequences. Herein we show that CodY represses its target genes by binding to specific DNA sequences upstream of the respective genes. A conserved target site was identified by analyzing upstream sequences of derepressed genes in a delta codY L. lactis MG1363 derivative, as identified in a DNA micro-array study. The present application furthermore discloses CodY target sequences from other gram-positive bacteria, like B. subtilis and Streptococcus. Hence, the invention provides CodY target sequences that may be used in different applications to repress or derepress gene expression.

AB - The invention relates to the field of biochemistry, molecular biology and food production. More in particular, the invention relates to methods and means for regulating gene expression. Even more in particular, the invention relates to CodY target sequences. Herein we show that CodY represses its target genes by binding to specific DNA sequences upstream of the respective genes. A conserved target site was identified by analyzing upstream sequences of derepressed genes in a delta codY L. lactis MG1363 derivative, as identified in a DNA micro-array study. The present application furthermore discloses CodY target sequences from other gram-positive bacteria, like B. subtilis and Streptococcus. Hence, the invention provides CodY target sequences that may be used in different applications to repress or derepress gene expression.

M3 - Patent

M1 - WO2005003354

Y2 - 2004/07/02

ER -

Methods and means for regulating gene expression

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