Abstract
Chlorate-resistant mutants of Arabidopsis thaliana were isolated in order to
find nitrate reductase-less mutants. It appeared that chlorate resistance in higher
plants can arise by mutations concerning two different mechanisms: (5) a lower
reduction rate of chlorate due to a lower level of nitrate reductase activity; (2) a
lower increase in content of chlorate and/or chlorite and of chloride after chlorate
treatment. One mutant of the first type and two mutants of the second type are
described. The nitrate reductase-less mutant grows poorly on a medium with nitrate
as the only nitrogen source but is not blocked in the uptake of nitrate. Both the other
mutants exhibit a nitrate reductase activity equal to or higher than that of the wild
type, but probably have a much lowered uptake of chlorate. The latter two mutants
belong to the same complementation group, whereas the nitrate reductase-less mutant
belongs to a different group.
find nitrate reductase-less mutants. It appeared that chlorate resistance in higher
plants can arise by mutations concerning two different mechanisms: (5) a lower
reduction rate of chlorate due to a lower level of nitrate reductase activity; (2) a
lower increase in content of chlorate and/or chlorite and of chloride after chlorate
treatment. One mutant of the first type and two mutants of the second type are
described. The nitrate reductase-less mutant grows poorly on a medium with nitrate
as the only nitrogen source but is not blocked in the uptake of nitrate. Both the other
mutants exhibit a nitrate reductase activity equal to or higher than that of the wild
type, but probably have a much lowered uptake of chlorate. The latter two mutants
belong to the same complementation group, whereas the nitrate reductase-less mutant
belongs to a different group.
| Original language | English |
|---|---|
| Pages (from-to) | 175-185 |
| Number of pages | 11 |
| Journal | Mutation Research |
| Volume | 19 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1973 |