Genome of the house fly, Musca domestica L., a global vector of diseases with adaptations to a septic environment

Jeffrey G Scott; Wesley C Warren; Leo W Beukeboom; Daniel Bopp; Andrew G Clark; Sarah D Giers; Monika Hediger; Andrew K Jones; Shinji Kasai; Cheryl A Leichter; Ming Li; Richard P Meisel; Patrick Minx; Terence D Murphy; David R Nelson; William R Reid; Frank D Rinkevich; Hugh M Robertson; Timothy B Sackton; David B Sattelle; Francoise Thibaud-Nissen; Chad Tomlinson; Louis van de Zande; Kimberly Walden; Richard K Wilson; Nannan Liu

doi:10.1186/s13059-014-0466-3

Genome of the house fly, Musca domestica L., a global vector of diseases with adaptations to a septic environment

Jeffrey G Scott, Wesley C Warren, Leo W Beukeboom, Daniel Bopp, Andrew G Clark, Sarah D Giers, Monika Hediger, Andrew K Jones, Shinji Kasai, Cheryl A Leichter, Ming Li, Richard P Meisel, Patrick Minx, Terence D Murphy, David R Nelson, William R Reid, Frank D Rinkevich, Hugh M Robertson, Timothy B Sackton, David B SattelleFrancoise Thibaud-Nissen, Chad Tomlinson, Louis van de Zande, Kimberly Walden, Richard K Wilson, Nannan Liu

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Abstract

BACKGROUND: Adult house flies, Musca domestica L., are mechanical vectors of more than 100 devastating diseases that have severe consequences for human and animal health. House fly larvae play a vital role as decomposers of animal wastes, and thus live in intimate association with many animal pathogens.

RESULTS: We have sequenced and analyzed the genome of the house fly using DNA from female flies. The sequenced genome is 691 Mb. Compared with Drosophila melanogaster, the genome contains a rich resource of shared and novel protein coding genes, a significantly higher amount of repetitive elements, and substantial increases in copy number and diversity of both the recognition and effector components of the immune system, consistent with life in a pathogen-rich environment. There are 146 P450 genes, plus 11 pseudogenes, in M. domestica, representing a significant increase relative to D. melanogaster and suggesting the presence of enhanced detoxification in house flies. Relative to D. melanogaster, M. domestica has also evolved an expanded repertoire of chemoreceptors and odorant binding proteins, many associated with gustation.

CONCLUSIONS: This represents the first genome sequence of an insect that lives in intimate association with abundant animal pathogens. The house fly genome provides a rich resource for enabling work on innovative methods of insect control, for understanding the mechanisms of insecticide resistance, genetic adaptation to high pathogen loads, and for exploring the basic biology of this important pest. The genome of this species will also serve as a close out-group to Drosophila in comparative genomic studies.

Original language	English
Article number	466
Number of pages	16
Journal	Genome Biology
Volume	15
Issue number	10
DOIs	https://doi.org/10.1186/s13059-014-0466-3
Publication status	Published - 14-Oct-2014

Keywords

BIASED GENE-EXPRESSION
ACETYLCHOLINE-RECEPTOR SUBUNITS
IONOTROPIC GLUTAMATE RECEPTORS
MULTIPLE SEQUENCE ALIGNMENT
DROSOPHILA-MELANOGASTER
MAXIMUM-LIKELIHOOD
LINKAGE GROUPS
NSECTICIDE RESISTANCE
CHEMOSENSORY RECEPTORS
ANTIMICROBIAL PEPTIDE

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Genome of the house fly, Musca domestica L., a global vector of diseases with adaptations to a septic environmentFinal publisher's version, 1.75 MBLicence: CC BY

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Testing theories of sex determination and sex chromosome evolution with houseflies
Beukeboom, L. (PI), Jacobus Mgn Van De Zande, L. (PI) & Sharma, A. (PhD student)
16/01/2013 → 16/01/2017
Project: Research

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Scott, J. G., Warren, W. C., Beukeboom, L. W., Bopp, D., Clark, A. G., Giers, S. D., Hediger, M., Jones, A. K., Kasai, S., Leichter, C. A., Li, M., Meisel, R. P., Minx, P., Murphy, T. D., Nelson, D. R., Reid, W. R., Rinkevich, F. D., Robertson, H. M., Sackton, T. B., ... Liu, N. (2014). Genome of the house fly, Musca domestica L., a global vector of diseases with adaptations to a septic environment. Genome Biology, 15(10), Article 466. https://doi.org/10.1186/s13059-014-0466-3

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title = "Genome of the house fly, Musca domestica L., a global vector of diseases with adaptations to a septic environment",

abstract = "BACKGROUND: Adult house flies, Musca domestica L., are mechanical vectors of more than 100 devastating diseases that have severe consequences for human and animal health. House fly larvae play a vital role as decomposers of animal wastes, and thus live in intimate association with many animal pathogens.RESULTS: We have sequenced and analyzed the genome of the house fly using DNA from female flies. The sequenced genome is 691 Mb. Compared with Drosophila melanogaster, the genome contains a rich resource of shared and novel protein coding genes, a significantly higher amount of repetitive elements, and substantial increases in copy number and diversity of both the recognition and effector components of the immune system, consistent with life in a pathogen-rich environment. There are 146 P450 genes, plus 11 pseudogenes, in M. domestica, representing a significant increase relative to D. melanogaster and suggesting the presence of enhanced detoxification in house flies. Relative to D. melanogaster, M. domestica has also evolved an expanded repertoire of chemoreceptors and odorant binding proteins, many associated with gustation.CONCLUSIONS: This represents the first genome sequence of an insect that lives in intimate association with abundant animal pathogens. The house fly genome provides a rich resource for enabling work on innovative methods of insect control, for understanding the mechanisms of insecticide resistance, genetic adaptation to high pathogen loads, and for exploring the basic biology of this important pest. The genome of this species will also serve as a close out-group to Drosophila in comparative genomic studies.",

keywords = "BIASED GENE-EXPRESSION, ACETYLCHOLINE-RECEPTOR SUBUNITS, IONOTROPIC GLUTAMATE RECEPTORS, MULTIPLE SEQUENCE ALIGNMENT, DROSOPHILA-MELANOGASTER, MAXIMUM-LIKELIHOOD, LINKAGE GROUPS, NSECTICIDE RESISTANCE, CHEMOSENSORY RECEPTORS, ANTIMICROBIAL PEPTIDE",

author = "Scott, {Jeffrey G} and Warren, {Wesley C} and Beukeboom, {Leo W} and Daniel Bopp and Clark, {Andrew G} and Giers, {Sarah D} and Monika Hediger and Jones, {Andrew K} and Shinji Kasai and Leichter, {Cheryl A} and Ming Li and Meisel, {Richard P} and Patrick Minx and Murphy, {Terence D} and Nelson, {David R} and Reid, {William R} and Rinkevich, {Frank D} and Robertson, {Hugh M} and Sackton, {Timothy B} and Sattelle, {David B} and Francoise Thibaud-Nissen and Chad Tomlinson and {van de Zande}, Louis and Kimberly Walden and Wilson, {Richard K} and Nannan Liu",

year = "2014",

month = oct,

day = "14",

doi = "10.1186/s13059-014-0466-3",

language = "English",

volume = "15",

journal = "Genome Biology",

issn = "1465-6906",

publisher = "BMC",

number = "10",

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Scott, JG, Warren, WC, Beukeboom, LW, Bopp, D, Clark, AG, Giers, SD, Hediger, M, Jones, AK, Kasai, S, Leichter, CA, Li, M, Meisel, RP, Minx, P, Murphy, TD, Nelson, DR, Reid, WR, Rinkevich, FD, Robertson, HM, Sackton, TB, Sattelle, DB, Thibaud-Nissen, F, Tomlinson, C, van de Zande, L, Walden, K, Wilson, RK & Liu, N 2014, 'Genome of the house fly, Musca domestica L., a global vector of diseases with adaptations to a septic environment', Genome Biology, vol. 15, no. 10, 466. https://doi.org/10.1186/s13059-014-0466-3

TY - JOUR

T1 - Genome of the house fly, Musca domestica L., a global vector of diseases with adaptations to a septic environment

AU - Scott, Jeffrey G

AU - Warren, Wesley C

AU - Beukeboom, Leo W

AU - Bopp, Daniel

AU - Clark, Andrew G

AU - Giers, Sarah D

AU - Hediger, Monika

AU - Jones, Andrew K

AU - Kasai, Shinji

AU - Leichter, Cheryl A

AU - Li, Ming

AU - Meisel, Richard P

AU - Minx, Patrick

AU - Murphy, Terence D

AU - Nelson, David R

AU - Reid, William R

AU - Rinkevich, Frank D

AU - Robertson, Hugh M

AU - Sackton, Timothy B

AU - Sattelle, David B

AU - Thibaud-Nissen, Francoise

AU - Tomlinson, Chad

AU - van de Zande, Louis

AU - Walden, Kimberly

AU - Wilson, Richard K

AU - Liu, Nannan

PY - 2014/10/14

Y1 - 2014/10/14

N2 - BACKGROUND: Adult house flies, Musca domestica L., are mechanical vectors of more than 100 devastating diseases that have severe consequences for human and animal health. House fly larvae play a vital role as decomposers of animal wastes, and thus live in intimate association with many animal pathogens.RESULTS: We have sequenced and analyzed the genome of the house fly using DNA from female flies. The sequenced genome is 691 Mb. Compared with Drosophila melanogaster, the genome contains a rich resource of shared and novel protein coding genes, a significantly higher amount of repetitive elements, and substantial increases in copy number and diversity of both the recognition and effector components of the immune system, consistent with life in a pathogen-rich environment. There are 146 P450 genes, plus 11 pseudogenes, in M. domestica, representing a significant increase relative to D. melanogaster and suggesting the presence of enhanced detoxification in house flies. Relative to D. melanogaster, M. domestica has also evolved an expanded repertoire of chemoreceptors and odorant binding proteins, many associated with gustation.CONCLUSIONS: This represents the first genome sequence of an insect that lives in intimate association with abundant animal pathogens. The house fly genome provides a rich resource for enabling work on innovative methods of insect control, for understanding the mechanisms of insecticide resistance, genetic adaptation to high pathogen loads, and for exploring the basic biology of this important pest. The genome of this species will also serve as a close out-group to Drosophila in comparative genomic studies.

AB - BACKGROUND: Adult house flies, Musca domestica L., are mechanical vectors of more than 100 devastating diseases that have severe consequences for human and animal health. House fly larvae play a vital role as decomposers of animal wastes, and thus live in intimate association with many animal pathogens.RESULTS: We have sequenced and analyzed the genome of the house fly using DNA from female flies. The sequenced genome is 691 Mb. Compared with Drosophila melanogaster, the genome contains a rich resource of shared and novel protein coding genes, a significantly higher amount of repetitive elements, and substantial increases in copy number and diversity of both the recognition and effector components of the immune system, consistent with life in a pathogen-rich environment. There are 146 P450 genes, plus 11 pseudogenes, in M. domestica, representing a significant increase relative to D. melanogaster and suggesting the presence of enhanced detoxification in house flies. Relative to D. melanogaster, M. domestica has also evolved an expanded repertoire of chemoreceptors and odorant binding proteins, many associated with gustation.CONCLUSIONS: This represents the first genome sequence of an insect that lives in intimate association with abundant animal pathogens. The house fly genome provides a rich resource for enabling work on innovative methods of insect control, for understanding the mechanisms of insecticide resistance, genetic adaptation to high pathogen loads, and for exploring the basic biology of this important pest. The genome of this species will also serve as a close out-group to Drosophila in comparative genomic studies.

KW - BIASED GENE-EXPRESSION

KW - ACETYLCHOLINE-RECEPTOR SUBUNITS

KW - IONOTROPIC GLUTAMATE RECEPTORS

KW - MULTIPLE SEQUENCE ALIGNMENT

KW - DROSOPHILA-MELANOGASTER

KW - MAXIMUM-LIKELIHOOD

KW - LINKAGE GROUPS

KW - NSECTICIDE RESISTANCE

KW - CHEMOSENSORY RECEPTORS

KW - ANTIMICROBIAL PEPTIDE

U2 - 10.1186/s13059-014-0466-3

DO - 10.1186/s13059-014-0466-3

M3 - Article

C2 - 25315136

SN - 1465-6906

VL - 15

JO - Genome Biology

JF - Genome Biology

IS - 10

M1 - 466

ER -

Genome of the house fly, Musca domestica L., a global vector of diseases with adaptations to a septic environment

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Keywords

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Testing theories of sex determination and sex chromosome evolution with houseflies

Cite this