Development of a proxy for past surface UV-B irradiation: A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores

Peter Blokker; Dan Yeloff; Peter Boelen; Rob A Broekman; Jelte Rozema

doi:10.1021/ac050696k

Development of a proxy for past surface UV-B irradiation: A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores

Peter Blokker^*, Dan Yeloff, Peter Boelen, Rob A Broekman, Jelte Rozema

^*Corresponding author for this work

Ocean Ecosystems

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Abstract

A method was developed for the analysis of the UV-absorbing sporopollenin monomers p-coumaric acid and ferulic acid in very low numbers of pollen. This enables the analysis of pollen or spores from cultured plants, from herbarium collections, and from sediment, soil, and peat cores. The method involves thermally assisted hydrolysis and methylation using tetramethylammonium hydroxide combined with gas chromatography and mass spectrometry. Pyrolysis, gas chromatographic, and mass spectrometric conditions were optimized for the analysis of minimal amounts of pollen. The method has a detection limit of approximately 60 fresh pollen of Alnus glutinosa and a relative standard deviation of approximately 10% between 100 and 600 pollen.

Original language	English
Pages (from-to)	6026-6031
Number of pages	6
Journal	Analytical Chemistry
Volume	77
Issue number	18
DOIs	https://doi.org/10.1021/ac050696k
Publication status	Published - 15-Sept-2005

Keywords

Alnus
Chromatography, Gas
Hydrolysis
Mass Spectrometry
Methylation
Microscopy, Electron, Scanning
Pollen
Seeds
Spores
Temperature
Ultraviolet Rays

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Development of a proxy for past surface UV-B irradiation A thermally
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title = "Development of a proxy for past surface UV-B irradiation: A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores",

abstract = "A method was developed for the analysis of the UV-absorbing sporopollenin monomers p-coumaric acid and ferulic acid in very low numbers of pollen. This enables the analysis of pollen or spores from cultured plants, from herbarium collections, and from sediment, soil, and peat cores. The method involves thermally assisted hydrolysis and methylation using tetramethylammonium hydroxide combined with gas chromatography and mass spectrometry. Pyrolysis, gas chromatographic, and mass spectrometric conditions were optimized for the analysis of minimal amounts of pollen. The method has a detection limit of approximately 60 fresh pollen of Alnus glutinosa and a relative standard deviation of approximately 10% between 100 and 600 pollen.",

keywords = "Alnus, Chromatography, Gas, Hydrolysis, Mass Spectrometry, Methylation, Microscopy, Electron, Scanning, Pollen, Seeds, Spores, Temperature, Ultraviolet Rays",

author = "Peter Blokker and Dan Yeloff and Peter Boelen and Broekman, {Rob A} and Jelte Rozema",

year = "2005",

month = sep,

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doi = "10.1021/ac050696k",

language = "English",

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journal = "Analytical Chemistry",

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T2 - A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores

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AU - Yeloff, Dan

AU - Boelen, Peter

AU - Broekman, Rob A

AU - Rozema, Jelte

PY - 2005/9/15

Y1 - 2005/9/15

N2 - A method was developed for the analysis of the UV-absorbing sporopollenin monomers p-coumaric acid and ferulic acid in very low numbers of pollen. This enables the analysis of pollen or spores from cultured plants, from herbarium collections, and from sediment, soil, and peat cores. The method involves thermally assisted hydrolysis and methylation using tetramethylammonium hydroxide combined with gas chromatography and mass spectrometry. Pyrolysis, gas chromatographic, and mass spectrometric conditions were optimized for the analysis of minimal amounts of pollen. The method has a detection limit of approximately 60 fresh pollen of Alnus glutinosa and a relative standard deviation of approximately 10% between 100 and 600 pollen.

AB - A method was developed for the analysis of the UV-absorbing sporopollenin monomers p-coumaric acid and ferulic acid in very low numbers of pollen. This enables the analysis of pollen or spores from cultured plants, from herbarium collections, and from sediment, soil, and peat cores. The method involves thermally assisted hydrolysis and methylation using tetramethylammonium hydroxide combined with gas chromatography and mass spectrometry. Pyrolysis, gas chromatographic, and mass spectrometric conditions were optimized for the analysis of minimal amounts of pollen. The method has a detection limit of approximately 60 fresh pollen of Alnus glutinosa and a relative standard deviation of approximately 10% between 100 and 600 pollen.

KW - Alnus

KW - Chromatography, Gas

KW - Hydrolysis

KW - Mass Spectrometry

KW - Methylation

KW - Microscopy, Electron, Scanning

KW - Pollen

KW - Seeds

KW - Spores

KW - Temperature

KW - Ultraviolet Rays

U2 - 10.1021/ac050696k

DO - 10.1021/ac050696k

M3 - Article

C2 - 16159137

SN - 0003-2700

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SP - 6026

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JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 18

ER -

Development of a proxy for past surface UV-B irradiation: A thermally assisted hydrolysis and methylation py-GC/MS method for the analysis of pollen and spores

Abstract

Keywords

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