Degradation of 3-chlorobenzoate under low-oxygen conditions in pure and mixed cultures of the anoxygenic photoheterotroph Rhodopseudomonas palustris DCP3 and an aerobic Alcaligenes species

J Krooneman*, TMP Gomes, LJ Forney, JC Gottschal

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    19 Citations (Scopus)

    Abstract

    The presence or absence of molecular oxygen has been shown to play a crucial role in the degradability of haloaromatic compounds. In the present study, it was shown that anaerobic phototrophic 3-chlorobenzoate (3CBA) metabolism by Rhodopseudomonas palustris DCP3 is oxygen tolerant up to a concentration of 3 mu M O-2. Simultaneous oxidation of an additional carbon source permitted light-dependent anaerobic 3CBA degradation at oxygen input levels which, in the absence of such an additional compound, would result in inhibition of light-dependent dehalogenation. Experiments under the same experimental conditions with strain DCP3 in coculture with an aerobic 3CBA-utilizing heterotroph, Alcaligenes sp. strain L6, revealed that light-dependent dehalogenation of 3CBA did not occur. Under both oxygen limitation (O-2 <0.1 mu M) and low oxygen concentrations (3 mu M O-2), all the 3CBA was metabolized by the aerobic heterotroph. These data suggest that biodegradation of (halo)aromatics by photoheterotrophic bacteria such as R. palustris DCP3 may be restricted to anoxic photic environments.

    Original languageEnglish
    Pages (from-to)131-137
    Number of pages7
    JournalApplied and environmental microbiology
    Volume65
    Issue number1
    Publication statusPublished - Jan-1999

    Keywords

    • ANAEROBIC DEGRADATION
    • HYPOXIC ENVIRONMENTS
    • GROWTH-CONDITIONS
    • BENZOIC-ACID
    • BACTERIA
    • METABOLISM
    • BIODEGRADATION
    • FERMENTATION
    • PATHWAY
    • NITRATE

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