Abstract
The development of single-molecule tools has significantly impacted the way we think about biochemical processes. Watching a single protein in action allows us to observe kinetic details and rare subpopulations that are hidden in ensemble-averaging techniques. I will discuss here the pros and cons of the single-molecule approach in studying ligand binding in macromolecular systems and how these techniques can be applied to characterize the behavior of large multicomponent biochemical systems.
| Original language | English |
|---|---|
| Pages (from-to) | 440-443 |
| Number of pages | 4 |
| Journal | Nature Chemical Biology |
| Volume | 4 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2008 |