Comparison of different culture media for isolation of Chlamydia trachomatis by cell culture on HeLa cells

P Herbrink; M Zuyderwijk-Zwinkels; B Niesters; J Wagenvoort; W Van Dijk

doi:10.1007/BF01975820

Comparison of different culture media for isolation of Chlamydia trachomatis by cell culture on HeLa cells

P Herbrink, M Zuyderwijk-Zwinkels, B Niesters, J Wagenvoort, W Van Dijk

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Abstract

The isolation yield and number of Chlamydia trachomatis inclusions was compared using DEAE-dextran pretreated HeLa cells cultured in four different media: Eagle's minimal essential medium (EMEM) with and without cycloheximide and Dulbecco's modification of EMEM (DMEM) with and without cycloheximide. Using DMEM without cycloheximide the number of inclusions was significantly higher than using EMEM without cycloheximide. In addition, the size of the inclusions was greatly enhanced. Use of DMEM or EMEM with cycloheximide yielded results comparable to those obtained with DMEM without cycloheximide.

Original language	English
Pages (from-to)	656-9
Number of pages	4
Journal	European Journal of Clinical Microbiology & Infectious Diseases
Volume	10
Issue number	8
DOIs	https://doi.org/10.1007/BF01975820
Publication status	Published - Aug-1991
Externally published	Yes

Keywords

Bacteriological Techniques
Chlamydia trachomatis
Culture Media
Cycloheximide
HeLa Cells
Humans
Retrospective Studies

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title = "Comparison of different culture media for isolation of Chlamydia trachomatis by cell culture on HeLa cells",

abstract = "The isolation yield and number of Chlamydia trachomatis inclusions was compared using DEAE-dextran pretreated HeLa cells cultured in four different media: Eagle's minimal essential medium (EMEM) with and without cycloheximide and Dulbecco's modification of EMEM (DMEM) with and without cycloheximide. Using DMEM without cycloheximide the number of inclusions was significantly higher than using EMEM without cycloheximide. In addition, the size of the inclusions was greatly enhanced. Use of DMEM or EMEM with cycloheximide yielded results comparable to those obtained with DMEM without cycloheximide.",

keywords = "Bacteriological Techniques, Chlamydia trachomatis, Culture Media, Cycloheximide, HeLa Cells, Humans, Retrospective Studies",

author = "P Herbrink and M Zuyderwijk-Zwinkels and B Niesters and J Wagenvoort and {Van Dijk}, W",

year = "1991",

month = aug,

doi = "10.1007/BF01975820",

language = "English",

volume = "10",

pages = "656--9",

journal = "European Journal of Clinical Microbiology & Infectious Diseases",

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TY - JOUR

T1 - Comparison of different culture media for isolation of Chlamydia trachomatis by cell culture on HeLa cells

AU - Herbrink, P

AU - Zuyderwijk-Zwinkels, M

AU - Niesters, B

AU - Wagenvoort, J

AU - Van Dijk, W

PY - 1991/8

Y1 - 1991/8

N2 - The isolation yield and number of Chlamydia trachomatis inclusions was compared using DEAE-dextran pretreated HeLa cells cultured in four different media: Eagle's minimal essential medium (EMEM) with and without cycloheximide and Dulbecco's modification of EMEM (DMEM) with and without cycloheximide. Using DMEM without cycloheximide the number of inclusions was significantly higher than using EMEM without cycloheximide. In addition, the size of the inclusions was greatly enhanced. Use of DMEM or EMEM with cycloheximide yielded results comparable to those obtained with DMEM without cycloheximide.

AB - The isolation yield and number of Chlamydia trachomatis inclusions was compared using DEAE-dextran pretreated HeLa cells cultured in four different media: Eagle's minimal essential medium (EMEM) with and without cycloheximide and Dulbecco's modification of EMEM (DMEM) with and without cycloheximide. Using DMEM without cycloheximide the number of inclusions was significantly higher than using EMEM without cycloheximide. In addition, the size of the inclusions was greatly enhanced. Use of DMEM or EMEM with cycloheximide yielded results comparable to those obtained with DMEM without cycloheximide.

KW - Bacteriological Techniques

KW - Chlamydia trachomatis

KW - Culture Media

KW - Cycloheximide

KW - HeLa Cells

KW - Humans

KW - Retrospective Studies

U2 - 10.1007/BF01975820

DO - 10.1007/BF01975820

M3 - Article

C2 - 1748120

SN - 0934-9723

VL - 10

SP - 656

EP - 659

JO - European Journal of Clinical Microbiology & Infectious Diseases

JF - European Journal of Clinical Microbiology & Infectious Diseases

IS - 8

ER -

Comparison of different culture media for isolation of Chlamydia trachomatis by cell culture on HeLa cells

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