Characterisation of twenty-one European badger (Meles meles) microsatellite loci facilitates the discrimination of second-order relatives

Geetha Annavi; Deborah A. Dawson; Gavin J. Horsburgh; Carolyn Greig; Hannah L. Dugdale; Chris Newman; David W. Macdonald; Terry Burke

doi:10.1007/s12686-011-9392-9

Characterisation of twenty-one European badger (Meles meles) microsatellite loci facilitates the discrimination of second-order relatives

Geetha Annavi^*, Deborah A. Dawson, Gavin J. Horsburgh, Carolyn Greig, Hannah L. Dugdale, Chris Newman, David W. Macdonald, Terry Burke

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The European badger (Meles meles) breeds plurally in lowland England and is important economically due to its link with bovine tuberculosis (Mycobacterium bovis) transmission. To understand disease transmission and facilitate effective management, it is vital to elucidate the social structure of badger groups. To improve parentage assignment and the discrimination of relatives, we isolated and characterised 21 polymorphic microsatellite loci in 24 individuals from Wytham Woods, Oxfordshire, UK. These 21 loci increased the discrimination power between full-siblings and half-siblings from 71 to 88%, when added to the existing 31 loci. Similarly, the combined non-exclusion probability increased from 3.0 x 10(-8) to 5.8 x 10(-13). Newly isolated Mel-592 (FR745854) was X-linked, based on the genotypes of 48 known-sex individuals and will enhance the genetic sex-typing of badgers.

Original language	English
Pages (from-to)	515-518
Number of pages	4
Journal	Conservation genetics resources
Volume	3
Issue number	3
DOIs	https://doi.org/10.1007/s12686-011-9392-9
Publication status	Published - Jul-2011

Keywords

European badger
Meles meles
Mustelidae
Microsatellite
Parentage
X-chromosome linked locus
EURASIAN BADGER
DNA
POPULATION
PATERNITY
MOVEMENT

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@article{b40ab4696f0c4fe5b4317342bafa87ca,

title = "Characterisation of twenty-one European badger (Meles meles) microsatellite loci facilitates the discrimination of second-order relatives",

abstract = "The European badger (Meles meles) breeds plurally in lowland England and is important economically due to its link with bovine tuberculosis (Mycobacterium bovis) transmission. To understand disease transmission and facilitate effective management, it is vital to elucidate the social structure of badger groups. To improve parentage assignment and the discrimination of relatives, we isolated and characterised 21 polymorphic microsatellite loci in 24 individuals from Wytham Woods, Oxfordshire, UK. These 21 loci increased the discrimination power between full-siblings and half-siblings from 71 to 88%, when added to the existing 31 loci. Similarly, the combined non-exclusion probability increased from 3.0 x 10(-8) to 5.8 x 10(-13). Newly isolated Mel-592 (FR745854) was X-linked, based on the genotypes of 48 known-sex individuals and will enhance the genetic sex-typing of badgers.",

keywords = "European badger, Meles meles, Mustelidae, Microsatellite, Parentage, X-chromosome linked locus, EURASIAN BADGER, DNA, POPULATION, PATERNITY, MOVEMENT",

author = "Geetha Annavi and Dawson, {Deborah A.} and Horsburgh, {Gavin J.} and Carolyn Greig and Dugdale, {Hannah L.} and Chris Newman and Macdonald, {David W.} and Terry Burke",

year = "2011",

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doi = "10.1007/s12686-011-9392-9",

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pages = "515--518",

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T1 - Characterisation of twenty-one European badger (Meles meles) microsatellite loci facilitates the discrimination of second-order relatives

AU - Annavi, Geetha

AU - Dawson, Deborah A.

AU - Horsburgh, Gavin J.

AU - Greig, Carolyn

AU - Dugdale, Hannah L.

AU - Newman, Chris

AU - Macdonald, David W.

AU - Burke, Terry

PY - 2011/7

Y1 - 2011/7

N2 - The European badger (Meles meles) breeds plurally in lowland England and is important economically due to its link with bovine tuberculosis (Mycobacterium bovis) transmission. To understand disease transmission and facilitate effective management, it is vital to elucidate the social structure of badger groups. To improve parentage assignment and the discrimination of relatives, we isolated and characterised 21 polymorphic microsatellite loci in 24 individuals from Wytham Woods, Oxfordshire, UK. These 21 loci increased the discrimination power between full-siblings and half-siblings from 71 to 88%, when added to the existing 31 loci. Similarly, the combined non-exclusion probability increased from 3.0 x 10(-8) to 5.8 x 10(-13). Newly isolated Mel-592 (FR745854) was X-linked, based on the genotypes of 48 known-sex individuals and will enhance the genetic sex-typing of badgers.

AB - The European badger (Meles meles) breeds plurally in lowland England and is important economically due to its link with bovine tuberculosis (Mycobacterium bovis) transmission. To understand disease transmission and facilitate effective management, it is vital to elucidate the social structure of badger groups. To improve parentage assignment and the discrimination of relatives, we isolated and characterised 21 polymorphic microsatellite loci in 24 individuals from Wytham Woods, Oxfordshire, UK. These 21 loci increased the discrimination power between full-siblings and half-siblings from 71 to 88%, when added to the existing 31 loci. Similarly, the combined non-exclusion probability increased from 3.0 x 10(-8) to 5.8 x 10(-13). Newly isolated Mel-592 (FR745854) was X-linked, based on the genotypes of 48 known-sex individuals and will enhance the genetic sex-typing of badgers.

KW - European badger

KW - Meles meles

KW - Mustelidae

KW - Microsatellite

KW - Parentage

KW - X-chromosome linked locus

KW - EURASIAN BADGER

KW - DNA

KW - POPULATION

KW - PATERNITY

KW - MOVEMENT

U2 - 10.1007/s12686-011-9392-9

DO - 10.1007/s12686-011-9392-9

M3 - Article

SN - 1877-7252

VL - 3

SP - 515

EP - 518

JO - Conservation genetics resources

JF - Conservation genetics resources

IS - 3

ER -

Characterisation of twenty-one European badger (Meles meles) microsatellite loci facilitates the discrimination of second-order relatives

Abstract

Keywords

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